Table 2

Detection of six selected genes by RT-qPCR from a total of 9 samples in the 1 and 10 cell category and 10 samples in the 5 cell category
# of samples detected by RT-qPCR Rank percentile (n = 38695 probes)
Gene 1-cell (n = 9) 5-cell (n = 10) 10-cell (n = 9) 1-cell (n = 9) 5-cell (n = 10) 10-cell (n = 9)
ACTB 7 7 9 100 100 100
GAPDH 8 10 9 94 94 90
YWHAZ 8 10 9 97 98 98
RPL13A 9 10 9 99 100 100

Detection was considered as having a Cq of <30. Rank percentile represents the average Alexa Fluor® 3 intensity from all oligonucleotides on the array that passed quality control for a given gene with outliers excluded using Dixon’s Q test. n = number of samples analyzed.

Welty et al.

Welty et al. BMC Molecular Biology 2013 14:6   doi:10.1186/1471-2199-14-6

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