Additional file 4.
Sucrose gradient analysis. Polysomal RNA (28S/18S ratio ~ 2) was separated from non-polysomal RNA (28S/18S ratio ≠2) by sucrose gradient centrifugation. Ratio of 18S and 28S rRNA was measured to obtain the polysome profile on Agilent 2100 Bioanalyzer. Fractions 5 to 7 contain non-polysomal RNA and fractions 9 to 11 polysomal RNA.
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Fromm-Dornieden et al. BMC Molecular Biology 2012 13:9 doi:10.1186/1471-2199-13-9