Binding of ELK-1 and EGR-1 to ZFP36 promoter. (A) MCF-7 cells were co-transfected with wild type ZFP36 and ZFP36 deletion mutant R5 (without -293 to -102 bp and +745 to +905 bp promoter fragment) and with or without pElk-VP16 (50 or 100 ng). 24 hrs after transfection luciferase activity was measured. Representative results from three independent experiments are shown and plotted as means ± SD (n = 3). (**P < 0,01). (B) Chromatin immunoprecipitation of transcription factors bound to ZFP36 promoter. Sonicated chromatin from MCF-7 cells grown in 10% FCS medium was immunoptrecipitated with either an ati-ELK-1 or anti-EGR-1 antibody or nonspecific IgG. PCR analysis of eluted DNA was performed using oligonucleotides specific for the distal region (upper panel) or proximal region (lower panel). 2% of input DNA is shown. The panels shown are inverted images of ethidium bromide-stained gels.
Florkowska et al. BMC Molecular Biology 2012 13:8 doi:10.1186/1471-2199-13-8