Table 1

A comparison of oligonucleotide-based strategies for polyQ diseases based on the desired properties of the reagents

PROPERTIES OF REAGENT

STRATEGIES

target specificity

allele selectivity

universality

stability in cells

delivery in shRNA


Specific silencing by RNAi

uncertain

no

uncertain

uncertain

yes


Replacement strategy

uncertain

uncertain

uncertain

uncertain

yes


SNP targeting

uncertain

yes

no

uncertain

yes


Repeat targeting by siRNA

no

no

yes

uncertain

yes


Repeat targeting by miRNA

uncertain

yes

yes

uncertain

yes


Repeat targeting by ASO

uncertain

yes

yes

yes

no


Fiszer et al. BMC Molecular Biology 2012 13:6   doi:10.1186/1471-2199-13-6

Open Data