Additional file 5.

Figure S4. Difficulties in the RT-PCR including the amplification of the expanded repeat tract. The RT-PCR analysis of the HTT alleles' transcript levels in the HD fibroblasts (GM04281) at 24 h after transfection with 10 nM control siRNA (C) or RNA (CUG)7. The two lanes on the left represent RT-PCR performed in standard conditions. The two lanes on the right include RNA purification from the fraction of short RNAs before RT-PCR.

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Fiszer et al. BMC Molecular Biology 2012 13:6   doi:10.1186/1471-2199-13-6