Additional file 1.

Figure S1. The activity of siRNAs targeting both alleles of HTT. (A) The target sites and nucleotide sequences of siRNAs directed at the mRNA of the HTT gene (lowercase for RNA, uppercase for DNA). (B) The RT-PCR analysis of HTT expression at the transcript level in the HD fibroblasts (GM04281) transfected with 50 nM siRNA HD1 at the indicated time points. (C) The western blot analysis of the huntingtin levels for the experiment described in (B). (D) The RT-PCR analysis of the HTT transcript levels in the HD fibroblasts (GM04281) at 72 h after transfection with 50 nM siRNAs HD1, HD2 and HD3. (E) The western blot analysis of the huntingtin levels in the HD fibroblasts (GM04281) at 72 h after transfection with 2, 10 or 50 nM siRNA HD1. C - the reference bar indicates the HTT expression level in the cells transfected with control siRNA. In the graphs, the signal intensities were normalized to GAPDH mRNA or protein levels and compared using a one-sample t-test. The error bars represent standard deviations. The P-value is indicated by asterisks (* p < 0.001; ** 0.001 < p < 0.05).

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Fiszer et al. BMC Molecular Biology 2012 13:6   doi:10.1186/1471-2199-13-6