Proto-silencer and replicator activity of ARS305 and ARS605. A. The ARSs and their mutant derivatives (shown on the left) were integrated in the VIIL telomere in URA3-ACS-B1-tel orientation. The WTW motifs are underlined. The mutations are depicted by small letters. The silencer activity of the constructs was assessed as % FOAR cells and plotted. Data is from Table 1. Statistical significance (p values) of the difference between the non-mutated construct and some of the mutants are shown next to each bar. The p values for the remaining constructs are significantly above 0.05 and are not shown. B. The ARSs and their mutant derivatives were cloned between CEN4 and URA3 to produce mini-chromosomes as indicated on the top of the figure. All mini-chromosomes were transformed in W303 cells and loss per generation was calculated. Data is from Table 1.
Chisamore-Robert et al. BMC Molecular Biology 2012 13:34 doi:10.1186/1471-2199-13-34