Amplification by RT-PCR of the long 5′UTR MDR1 cDNA.A. Diagram showing two ABCB1 promoters and the MDR1 mRNA isoform produced by each promoter. B. RT-PCR amplification with F1 and R1 primers of the long 5′ UTR of the MDR1 cDNA. Lane 1. Molecular markers.Lanes 2–7. IMIM-PC-1, IMIM-PC-2, RWP-1, Hs 766 T, BxPC-3 and PANC-1 cell lines. Lane 8.y 9, K-562/Adr and HTC-15 (positive controls).
Balaguer et al. BMC Molecular Biology 2012 13:25 doi:10.1186/1471-2199-13-25