Table 3

Analysis of the polysome profile obtained for the wild type and theΔyhr087wstrains
time (min) 0 15 30 45 60
BY4742 P (1) 77.12 ± 3.67 40.37 ± 1.60 64.65 ± 3.54 75.07 ± 1.95 75.74 ± 2.98
P/(F + M)(2) 3.43 ± 0.71 0.68 ± 0.04 1.84 ± 0.28 3.03 ± 0.33 3.15 ± 0.51
60/40 (3) 1.49 ± 0.19 1.73 ± 0.13 1.37 ± 0.39 1.23 ± 0.06 1.55 ± 0.26
Δyhr087w P (1) 76.34 ± 2.89 34.06 ± 2.31 59.32 ± 0.55 69.33 ± 1.46 75.25 ± 0.01
P/(F + M) (2) 3.26 ± 0.52 0.52 ± 0.05 1.46 ± 0.03 2.27 ± 0.16 4.54 ± 0.00
60/40 (3) 1.23 ± 0.14 1.66 ± 0.05 1.52 ± 0.28 1.15 ± 0.12 1.63 ± 0.12

(1) The data shown correspond to the percentage of the polysome area relative to the total area of polysomal plus free plus monosomal fractions. The amount of rRNA in each fraction was measured by UV detection at 260 nm. Three independent experiments were carried out: average and standard deviation are shown.

(2) Ratio between the area of the polysomal and free plus monosomal fractions.

(3) Ratio between the area corresponding to the 60 S and 40 S subunits.

Gomar-Alba et al.

Gomar-Alba et al. BMC Molecular Biology 2012 13:19   doi:10.1186/1471-2199-13-19

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