The translation of several stress-induced genes is affected in strainΔyhr087W. Cultures of the WT and Δ yhr087W strains growing exponentially in YPD were incubated for 45 min in 20% glucose and polysomal profiles were obtained. The fractions corresponding to free and monosomal (FM) and polysomal (P) were collected, RNA was isolated and cDNA was obtained. The expression of the indicated genes was analyzed by Real-Time RT-PCR using specific probes. Signals were normalized to Bacillus subtilis LysA mRNA, which was added to each group of fractions in equal amounts. The expression values shown are relative to those found in the ACT1 gene. The quantified signals from three independent replicates were used to determine the average change in P/FM ratios. Asterisks indicate statistically significant differences (* p-value < 0.05, ** p-value < 0.005).
Gomar-Alba et al. BMC Molecular Biology 2012 13:19 doi:10.1186/1471-2199-13-19