Sensitivity to translation elongation inhibitors of the mutants inYHR087Wand/or other genes encoding proteins that physically interact with Yhr087wp (according to the TAP experiment) and are involved, most of them, in the ribosomal function:STI1,SCP160,TIF4631(Panel A) andCDC33(Panel B). Serial dilutions were spotted on YPD, YPD cycloheximide (0.1 μg/mL) and YPD hygromycin (20 μg/mL or 10 μg/mL) plates, which were incubated for 48-72 h at 30°C. Incubation with mutants cdc33ts and Δyhr087w cdc33ts was carried out at 33°C until growth was detected.
Gomar-Alba et al. BMC Molecular Biology 2012 13:19 doi:10.1186/1471-2199-13-19