Yhr087wp partially associates with ribosomes. Whole cell extracts obtained from a strain containing an Yhr087wp-TAP-tagged protein were fractionated as described in the Materials and Methods section. The Figure shows the distribution of protein Yhr087wp between the cytoplasmic (CF) and ribosomal (RF) fractions by Western analysis. A sample corresponding to the whole cell extract (WCE) is also included. As a control of the fractionation procedure, the location of a cytosolic (α-tubulin) and a ribosomal protein (Rpl5p) was determined by Western analysis using specific antibodies against these proteins.
Gomar-Alba et al. BMC Molecular Biology 2012 13:19 doi:10.1186/1471-2199-13-19