Demonstration of the physical interaction between Yhr087wp, Sti1p, Tif4631p, Cdc33p and Tif32p by coimmunoprecipitation. Cells of the strains carrying a TAP-tagged version of the Yhr087wp and an HA-tagged version of Sti1p, Tif4631p, Cdc33p or Tif32p proteins were exponentially grown in YPD at 30°C. After a 90-minute incubation in YP20 at the same temperature, cells were lysed and Yhr087p was immunoprecipitated as described in the Materials and Methods section. This Figure shows the Western analysis carried out with the total protein samples (TP), the non-retained fraction (NR) and the retained fraction (R). Yhr087wp is detected with α-PAP antibody, while the other four proteins were visualized with α-HA antibody. The results of the control experiments carried out with a FY86-derived strain carrying HA-tagged versions of the Sti1p, Tif4631p, Cdc33p and Tif32p proteins are also shown.
Gomar-Alba et al. BMC Molecular Biology 2012 13:19 doi:10.1186/1471-2199-13-19