Illustration of the used VDR/Pim-1 variants. (A) In addition to full-length VDR the DNA Binding Domain (DBD: AA 16–125) and the Ligand Binding Domain (LBD: AA 111–427) of the VDR were subjected for Y2H analysis as bait molecules. They were tested with (B) full-length PIM-1 isoform 2 as well as the truncated version of PIM-1 (AA 135–313).
Maier et al. BMC Molecular Biology 2012 13:18 doi:10.1186/1471-2199-13-18