Additional file 4.

FACS analysis of differentiated T helper cells. FACS analysis shows cell-surface phenotype of differentiated Th1(A), Th2 (B), Th17 (C), nTreg (D) and iTreg (E) cells after intracellular staining with the corresponding antibodies, (A) anti-IFNg for Th1 cells, (B) anti-IL4 for Th2 cells, (C) anti-IL17 for Th17 cells or (D) anti-CD25 for nTreg and iTreg; all FACS analyses were gated at CD4+. All the differentiated cell subsets used in this study were purified by FACS sorting. All experiments were repeated at least 3 times

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Li et al. BMC Molecular Biology 2012 13:16   doi:10.1186/1471-2199-13-16