Additional file 1.

Shift from naive CD4+ T cells to memory and effector T subset cells during development. A. & B. FACS analysis shows cell-surface phenotype of unstimulated CD4+ T cells (NS) isolated from 2 week (A) and 16 week (B) old C57BL/6 mouse spleens gated at CD4+. Naive T cells are characterised by the presence of a CD44-CD62L+ phenotype, while memory and effector T subset cells display a CD44+CD62L+/- phenotype. C. - D. FACS analysis showing the cell-surface phenotype of PMA/I stimulated CD4+ T cells (4 h) isolated from 2 week (C) and 16 week (D) old C57BL/6 mice. Activated cells become CD62L negative. Every experiment was repeated at least 3 times

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Li et al. BMC Molecular Biology 2012 13:16   doi:10.1186/1471-2199-13-16