Figure 8.

Generation of 5-hydroxymethylcytosine at specific gene promoters in CD4+ T cells. A. - E. The MeDIP approach, using a 5-hydroxymethylcytosine (5hmC) antibody (Active Motif) that specifically recognizes 5-hydroxymethylcytosine, was applied to determine the DNA 5hmC levels at individual CpG dinucleotides located at the promoter regions of inducible genes in CD4+ T cells from mice of both age groups during PMA/I stimulation for 0, 0.5, 2 and 4 hours (A. - C.) and T cell differentiation (D. - E.); primers Il2 set B (A) and Il2 set C (B) are located at the Il2 promoter; C . primer Csf2 set A is located at the Csf2 promoter; primer sets A of Ifng and Il4 are located at the Ifng and Il4 promoters, respectively; primers Il17 set A and set B, Foxp3 set A and set B are located at the Il17 and Foxp3 promoters, respectively. The data were normalized to NS, and are shown as the mean and SEM of three independent experiments

Li et al. BMC Molecular Biology 2012 13:16   doi:10.1186/1471-2199-13-16
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