Figure 5.

mRNA expression of enzymes and co-factors involved in DNA demethylation in unstimulated CD4+ T cells in two age groups of mice. Total RNA was prepared from unstimulated CD4+ T cells in both age groups of mice. After reverse transcription, quantitative PCR analysis was performed on cDNA with primers designed to detect these enzymes and co-factors. mRNA expression of (A) DNA methyltransferases (Dnmt1, Dnmt3a), (B) deaminases (Apobec3 and Apobec4), glycosylases (Mbd4 and Tdg), Gadd45 family (Gadd45a, Gadd45b and Gadd45g) and (c) ten-eleven translocation family (Tet1, Tet2 and Tet3) was determined by real-time PCR. The gene expression data were normalized to the Ubc housekeeping gene, and are shown as the mean and SEM of three independent experiments

Li et al. BMC Molecular Biology 2012 13:16   doi:10.1186/1471-2199-13-16
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