Table 2

Detection of methylated DNA diluted in unmethylated DNA

Copies of methylated DNA

0

1.3

4

11.9

35.6

107

320

960


Detected methylated copies (RASSF1A)

0

0.5

4.9

11

41

142

328

832


Detected unmethylated copies (RASSF1A)

1748

2192

2297

2207

1832

2700

2285

2194


Detection of RASSF1A in a 3-fold dilution series of methylated DNA in a background of 2000 copies of DNA. Top row represents the calculated number of methylated copies of RASSF1A promoter. The following rows represent methylated and unmethylated copies respectively, detected by real-time PCR.

Pedersen et al. BMC Molecular Biology 2012 13:12   doi:10.1186/1471-2199-13-12

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