Functional analysis of putative transcription factor binding sites in the MTG16 promoter. Putative Sp1 (-425), GATA (-301) and MZF-1 (-251) sites in the -668-57 (-668) promoter were individually destroyed by site directed mutagenesis as decribed in Methods. Erythroid HEL/TF-1, erythroid/megakaryocytic UT-7 and megakaryocytic MEG-01cells were transfected and luciferase activity was determined. The pGL3/basic and pGL3/SV40-promoter are used as negative and positive control, respectively. The luciferase activity of mutated MTG16 promoter is normalized against luciferase activity of wildtype MTG16 promoter.
Ajore et al. BMC Molecular Biology 2012 13:11 doi:10.1186/1471-2199-13-11