Effect of Ste11ΔNp on Cmk2p, CMK2 and PMC1 reporter gene. (A) Effect of STE11ΔN over expression on FKS2, PMC1 and PRM5 mRNAs. Cells were grown as in Figure 4. (B) Genomic CMK2 with triple and double GFP confirmed by western blotting. (C) Electrophoresis mobility and abundance change of Cmk2-GFP induced by STE11ΔN. (D) CMK2-lacZ activation by STE11ΔN. (E) Lack of effect of STE11ΔN on PMC1-lacZ with Ca2+. (F) STE11ΔN stimulates PMC1-lacZ in a mpk1Δ mutant. Data were expressed as mean ± SE of at least three independent experiments. Statistical significance was computed by the unpaired Student's t test. *p < 0.05, **p < 0.01.
Wang et al. BMC Molecular Biology 2011 12:51 doi:10.1186/1471-2199-12-51