Kinetics of Hspa1b promoter induction after transfection with Lipofectine (CAT assay). Chloramphenicol acetyltransferase (CAT) activity was observed at different time points after transfection with Lipofectine of p950/CAT6 construct into B16F10 cells. Each experimental point was performed in duplicate. AcCM - acetylchloramphenicol, product of the enzymatic assay. CM - non-acetylated chloramphenicol, substrate of the assay.
Fiszer-Kierzkowska et al. BMC Molecular Biology 2011 12:27 doi:10.1186/1471-2199-12-27