The promoter of heat shock/stress gene Hspa1b is activated in physiological temperature when Lipofectin is used for transient transfection. Mouse melanoma B16F10 cells were transfected with pR70/GFP vector containing the rat Hspa1b gene promoter linked with EGFP reporter gene. Left panel: cells grown at 37°C for 48 h after lipofection. Right panel: cells grown at 37°C for 24 h, then heat-shocked for 45 min at 42,5°C followed by recovery for 24 h at 37°C. Upper row - EGFP expression observed in UV light; lower row - the same field of vision observed in visible light. Pictures were recorded with an image analyzer equipped with Hamamatsu Color Chilled 3 CCD. Magnification: 250 x.
Fiszer-Kierzkowska et al. BMC Molecular Biology 2011 12:27 doi:10.1186/1471-2199-12-27