Table 3

Effect of DNA on the rate of ATP and dATP hydrolysis catalyzed by DgeRecA.

Substrate

Rate of hydrolysis (μM/min)

absence of DNA

dsDNA

ssDNA

ssDNA and dsDNAa


ATP

not detected

not detected

26.8 ± 0.40

20.3 ± 0.36

dATP

not detected

not detected

31.6 ± 0.63

23.7 ± 0.43


Reactions conditions: 37°C, 2.27 mM ATP or dATP.

a DNA strand exchange conditions

Wanarska et al. BMC Molecular Biology 2011 12:17   doi:10.1186/1471-2199-12-17

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