Table 1

Summary of the purification of DgeRecA and DmuRecA proteins obtained from 1 L of E. coli BLR(DE3) culture.

Protein

Purification step

Total protein (mg)

Recovery of protein (%)


DgeRecA

Cell extract

760

100

Heat treatment

341

45

Fractogel EMD

121

16

DEAE column

ResourceQ column

75

10

MonoQ column

63

8


DmuRecA

Cell extract

510

100

Fractogel EMD

356

70

DEAE column

ResourceQ column

93

18

MonoQ column

50

10


Wanarska et al. BMC Molecular Biology 2011 12:17   doi:10.1186/1471-2199-12-17

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