Figure 5.

Binding of DgeRecA (A, B) and DmuRecA (C, D) to 5'-end fluorescein-labeled (dT)35-oligonucleotides in the presence of 10 mM Mg2+ at various temperatures - gel mobility shift assays. Panel A: lane 1 - oligo(dT)35, lane 2 - 25.0°C, lane - 3 - 31.1°C, lane 4 - 38.2°C, lane 5 - 45.3°C, lane 6 - 51.1°C, lane - 7 - 58.0°C, lane 8 - 65.3°C, lane 9 - 72.2°C, lane 10 - 75.0°C; panel B: lane 1 - oligo(dT)35, lane 2 - 55.0°C, lane 3 - 55.9°C, lane 4 - 57.1°C, lane 5 - 58.0°C, lane 6 - 59.4°C; panel C: lane 1 - oligo(dT)35, lane 2 - 25.0°C, lane - 3 - 25.9°C, lane 4 - 28.7°C, lane 5 - 33.1°C, lane 6 - 37.9°C, lane - 7 - 42.6°C, lane 8 - 47.4°C, lane 9 - 52.1°C, lane 10 - 56.9°C, lane 11 - 61.2°C, lane 12 - 64.1°C, lane 13 - 65.0°C; panel D: lane 1 - oligo(dT)35, lane 2 - 52.0°C, lane 3 - 53.2°C, lane 4 - 54.5°C, lane 5 - 55.6°C.

Wanarska et al. BMC Molecular Biology 2011 12:17   doi:10.1186/1471-2199-12-17
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