Effects of DgeRecA (A) and DmuRecA (B) proteins concentrations on the elution profiles of gel filtration. In panel A elution volume of 9.73 ml corresponding to the 14-meric protein; elution volume of 10.15 ml corresponding to the 12-meric protein; elution volumes of 10.60 and 12.52 ml corresponding to 10-meric and tetrameric proteins, respectively; elution volumes of 10.79 and 13.58 ml corresponding to 9-meric and trimeric proteins, respectively; elution volumes of 11.10 and 14.24 ml corresponding to octameric and dimeric proteins, respectively. In panel B elution volumes of 8.44, 8.48, 8.38 and 8.26 ml corresponding to protein agregates; elution volumes of 9.94, 13.22 and 14.29 ml corresponding to 13-meric, trimeric and dimeric proteins, respectively; elution volumes of 10.56, 13.30 and 14.35 ml corresponding to 10-meric, trimeric and dimeric proteins, respectively; elution volume of 11.12 ml corresponding to the octameric protein; elution volume of 13.24 ml corresponding to the trimeric protein; elution volume of 14.46 ml corresponding to the dimeric protein.
Wanarska et al. BMC Molecular Biology 2011 12:17 doi:10.1186/1471-2199-12-17