Figure 2.

SDS-PAGE analysis of the fractions obtained by expression and purification of DgeRecA and DmuRecA proteins. Lane 1 - LMW SDS Marker (Amersham Biosciences AB): 97, 66, 45, 30, 20.1 and 14.4 kDa, lane 2 - cell extract of the E. coli BLR(DE3) + pET-30Ek/LIC-DgeRecA, lane 3 - DgeRecA after heat treatment, lane 4 - DgeRecA after chromatography on Fractogel EMD DEAE column, lane 5 - DgeRecA after chromatography on ResourceQ column, lane 6 - DgeRecA after chromatography on MonoQ column, lane 7 - Unstained Protein Molecular Weight Marker (Fermentas): 116, 66.2, 45, 35, 25, 18.4 and 14.4 kDa, lane 8 - cell extract of E. coli BLR(DE3) + pET-30Ek/LIC-DmuRecA, lane 9 - DmuRecA after chromatography on Fractogel EMD DEAE column, lane 10 - DmuRecA after chromatography on ResourceQ column, lane 11 - DmuRecA after chromatography on MonoQ column.

Wanarska et al. BMC Molecular Biology 2011 12:17   doi:10.1186/1471-2199-12-17
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