Detection of heterogeneous miRNAs and pre-miRNAs. Examples of heterogeneous miRNAs and pre-miRNAs overexpressed in HEK 293T cells and detected with single-nucleotide resolution, as shown in the figure. A) miRNAs: miR-191, miR-496, miR-93 resolved in the range of ~17-25 nt. B) pre-miRNAs: pre-miR-191, pre-miR-496, and pre-miR-93 resolved in the range of ~60-70 nt. M-lanes denote the appropriate radiolabeled RNA oligonucleotide markers (ORNs); M1 denotes 17, 19, 21, 23, 25-nt ORNs for miRNA detection (A) and M2 denotes 60/61 and 63/64-nt ORNs for pre-miRNA detection (B). Quantitative representations of miRNA and pre-miRNA variants obtained from phosphorimaging analyses are shown schematically.
Koscianska et al. BMC Molecular Biology 2011 12:14 doi:10.1186/1471-2199-12-14