Correlation between high-resolution northern blotting and deep sequencing results. A) Appropriately cropped representative northern pictures for endogenous mouse neuromiRs: miR-9, miR-9*, miR-29, miR-124, miR-132, miR-137, and myomiRs: miR-1, and miR-206 are shown. B) Comparative analysis of the relative distribution of miRNA length variants. The percentage of the various length variants of mouse miR-9, miR-9*, miR-29, miR-124, miR-132, miR-137, miR-1, and miR-206 observed in our high-resolution northern blot detections are shown as black bars; equivalent miRNAs identified by deep sequencing are shown as gray bars. Relative shares of miRNA length variants (denoted as nt) are calculated in percentage (%). Standard errors are from twelve independent northern blot samples for miR-9, miR-9*, miR-29, miR-124, miR-132, miR-137, from nine samples for miR-1, and from three samples for miR-206. One-nucleotide discrepancy between northern blotting and deep sequencing results observed in the case of miR-206 may be due to a difference in the migration rate of this miRNA, which probably results from its nucleotide composition.
Koscianska et al. BMC Molecular Biology 2011 12:14 doi:10.1186/1471-2199-12-14