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Table 2
PCR primers used in this study.
mRNA	mRNA name	GenBank accession no.	Forward primer¹	Reverse primer¹	Amplicon Size [bp]	PCR efficiency [%]

ABL1	c-abl oncogene 1	NM_005157.3, NM_007313.2	GAGCACAGAGACACCACTGACG	GCTCATCTTCATTCAGGCCG	148	100.9
ACTB	β-actin	NM_001101.3	ATTGCCGACAGGATGCAGAA	GCTGATCCACATCTGCTGGAA	150	97.9
B2M	β-2-microglobulin	NM_004048.2	GCTCCGTGGCCTTAGCTGT	ACGTGAGTAAACCTGAATCTTTGGA	89	101.1
CD36	CD36	NM_001001548.2, NM_001001547.2, NM_000072.3, NM_001127443.1, NM_001127444.1	TCACTGCGACATGATTAATGGTACA	ACGTCGGATTCAAATACAGCATAGAT	126	99.2
EIF2B2	Eukaryotic translation initiation factor 2B2	NM_014239.2	TCCACCCCACTCATCGTCTG	TGGCAGGACTTCTTCAGGAGC	105	101.1
G6PD	Glucose-6-phosphate dehydrogenase	NM_000402.3, NM_001042351.1	CCGTCACCAAGAACATTCACG	GGACAGCCGGTCAGAGCTCT	107	98.7
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	NM_002046.3	CAACAGCGACACCCACTCCT	CACCCTGTTGCTGTAGCCAAA	115	101.7
GUSB	β-glucuronidase	NM_000181.3	AGCTCATTTGGAATTTTGCCG	GAGTGAAGATCCCCTTTTTATTCCC	81	101.8
H3F3A	H3 histone, family 3A	NM_002107.3	TGGCGCTCCGTGAAATTAGA	TGAGCAATTTCTCGCACCAGA	91	97.0
POLR2K	Polymerase (RNA) II (DNA-directed) polypeptide K	NM_005034.3	ACCCAGAAGGACGTTCAACCTC	TCTCTGCATCTGATTGGATCCC	107	98.9
PPARA	Peroxisome proliferator-activated receptor α	NM_001001928.2, NM_005036.4	AGCCCCTCCTCGGTGACTTAT	GCTTGAGTCGAATCGTTCGC	172	94.6
PPARD	Peroxisome proliferator-activated receptor δ	NM_006238.3, NM_177435.2	AGAACCGCAACAAGTGCCAG	GCATCCGACCAAAACGGA	87	99.1
PPARG	Peroxisome proliferator-activated receptor γ	NM_138712.3, NM_015869.4, NM_138711.3, NM_005037.5	TTCAGAAATGCCTTGCAGTGG	AGCTTCTCCTTCTCGGCCTG	79	100.6
PPIB	Peptidylprolyl isomerase B (cyclophilin B)	NM_000942.4	ATGGCAAGCATGTGGTGTTTG	CCCGGCTGTCTGTCTTGGT	84	96.8
PSMB2	Proteasome subunit, β type 2	NM_002794.3	ACGGCAGCAGCTAACTTCACA	TGGCCCTTCATGCTCATCA	108	101.9
PSMB6	Proteasome subunit, β type 6	NM_002798.1	GGAATCATCATCGCAGGCTG	CTGCCTTACCATCATACCCCC	81	101.6
QARS	Glutaminyl-tRNA synthetase	NM_005051.1	GAGCGTCTTGGATATTTCTCCGT	GCTTCCAGCTCACACCTTTCC	108	98.3
RPL37A	Ribosomal protein L37a	NM_000998.4	ATTGAAATCAGCCAGCACGC	AGGAACCACAGTGCCAGATCC	94	97.6
SRP14	Signal recognition particle 14 kDa	NM_003134.4	AGCACTGTGGTGAGCTCCAAG	TCAGCCCATCCATGTTAGCTCTA	82	95.1
TUBA1	α1-tubulin	NM_006009.2, NM_006082.2, NM_032704.3	GCACTACACCATTGGCAAGGA	AACCAGTTCCCCCACCAAAG	122	98.4
TXNRD1	Thioredoxin reductase 1	NM_003330.2, NM_182742.1, NM_182729.1, NM_182743.1, NM_001093771.1	CACAATTGGAATCCACCCTGTC	GCTTGCCCCAGAGCGC	73	99.2
UBE2D2	Ubiquitin-conjugating enzyme E2D 2	NM_003339.2	CCAGATGATCCTTTAGTGCCTGAG	ACATCGCATACTTCTGAGTCCATTC	100	102.5

¹In each case, forward and reverse primers are located in different exons.
Maeß et al. BMC Molecular Biology 2010 11:90 doi:10.1186/1471-2199-11-90

Table 2