Additional file 3.

Figure S2. Determination of the number of genes required for calculating GeNorm normalization factor. Variances of pairwise combined normalization factors were calculated in order to determine which genes had to be considered for inclusion into the GeNorm normalization factor. Each bar represents the variance of the normalization factors when an additional gene is included into the calculation; the starting set of normalization factors is calculated from the two most stable genes (ACTB and RPL37A). Further genes are included according to the stability ranking calculated previously; according to Vandesompele et al. further genes are recommended to be included until the variance is below 0.15 [1]. Reference [1] Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F: Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 2002, 3:research0034.1-0034.11.

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MaeƟ et al. BMC Molecular Biology 2010 11:90   doi:10.1186/1471-2199-11-90