Open Access Highly Accessed Research article

Sex-biased transcription enhancement by a 5' tethered Gal4-MOF histone acetyltransferase fusion protein in Drosophila

Anja H Schiemann1, Fang Li14, Vikki M Weake13, Esther J Belikoff14, Kent C Klemmer2, Stanley A Moore2 and Maxwell J Scott14*

Author Affiliations

1 Institute of Molecular BioSciences, Massey University, Private Bag 11222, Palmerston North, New Zealand

2 Dept. of Biochemistry, University of Saskatchewan, 107 Wiggins Rd., Saskatoon, Saskatchewan S7N 5E5, Canada

3 Stowers Institute for Medical Research, Kansas City, Missouri, USA

4 Department of Genetics, North Carolina State University, Campus Box 7614, Raleigh NC 27695-7614, USA

For all author emails, please log on.

BMC Molecular Biology 2010, 11:80  doi:10.1186/1471-2199-11-80

Published: 9 November 2010

Additional files

Additional file 1:

Figure S1. An X-linked UAS-RedStinger line is activated more strongly in females than males by Gal4-MOF. Gal4-MOF line S41 was crossed with UAS-RedStinger3 ([FlyBase ID FBst0008545]) and the third instar larval offspring were examined for nuclear red fluorescence.

Format: PNG Size: 516KB Download file

Open Data

Additional file 2:

Figure S2. H4K16ac is enriched at 3 × UAS-arm-lacZ and UAS-lacZ reporter genes in the presence of Gal4-MOF Independent ChIP experiments were performed with nuclei isolated from larvae that carry hsp83-Gal4-MOF and either UAS-lacZ or 3 × UAS-arm-lacZ transgenes. See main text for details

Format: PDF Size: 55KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data