Additional file 5.
Figure S4 Purified GST-Elf5 WT and the two DNA-binding deficient mutants. GST-Elf5 wildtype and GST-Elf5 mutants were purified using GST-agarose and eluted samples were run on a SDS-PAGE gel to assess purity and amount of the proteins. The WT1 and WT2 samples represent two independently purified fractions. The mutants, MT1 and MT2 were K to A substitution at amino acid 216 and R to A substitution at amino acid 219 respectively, of the mouse Elf5 protein.
Format: PDF Size: 362KB Download file
This file can be viewed with: Adobe Acrobat Reader
Escamilla-Hernandez et al. BMC Molecular Biology 2010 11:68 doi:10.1186/1471-2199-11-68