Open Access Highly Accessed Research article

Predator-induced defences in Daphnia pulex: Selection and evaluation of internal reference genes for gene expression studies with real-time PCR

Katina I Spanier1, Florian Leese1*, Christoph Mayer1, John K Colbourne3, Don Gilbert3, Michael E Pfrender2 and Ralph Tollrian1

Author Affiliations

1 Ruhr-University Bochum, Department of Animal Ecology, Evolution and Biodiversity, D-44780 Bochum, Germany

2 University of Notre Dame, Department of Biological Sciences, Notre Dame, IN 46556 USA

3 Indiana University, The Center for Genomics and Bioinformatics, Bloomington, IN 47405 USA

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BMC Molecular Biology 2010, 11:50  doi:10.1186/1471-2199-11-50

Published: 29 June 2010

Additional files

Additional file 1:

Results of the gene expression analysis with REST when using aTub (1a) and GAPDH (1b) as reference genes for expression normalization.

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