Additional file 8.
Western blot analysis to detect the overexpression of lrp on protein level. Transcription of the lrp gene was under the control of the bacteriorhodopsin (bop) promoter resulting in the KF203 (↑lrp) mutant. The bop promoter is maximally induced under light exposure and anaerobic conditions. Therefore wild type cells and cells from the Lrp-overexpression strain were grown anaerobically under light exposure and harvested at an OD600 of 0.8. For detection of the Lrp protein we used an antibody against Lrp. Proteins from strains as indicated in the figure were separated on a gradient gel (4-12%), blotted on a nitrocellulose membrane and finally subjected to an immune detection reaction with an antibody against Lrp. Low expression was observed for the wild type (lane 1), whereas significant overexpression was detected in the Lrp-overexpression strain (lane 2). Furthermore we tested the Δlrp deletion mutant, grown aerobically in the dark. Using an antibody against Lrp (lane 3) no signal could be obtained, as expected.
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Schwaiger et al. BMC Molecular Biology 2010 11:40 doi:10.1186/1471-2199-11-40