Additional file 11.
Scheme of integration of pKF204 into the genome of H. salinarum resulting in KF204 (↑lrpA1) mutant. The plasmid pKF204 contains a portion of the 5'end of the lrpA1 gene. The bop promoter Pbop (violet box) was inserted upstream of the truncated lrpA1 gene (black arrow). The plasmid contains a selection marker (MevR). After integration of pKF204 into the H. salinarum genome, only the lrpA1 gene downstream the bop promoter is functionally transcribed, while transcription product under the native promoter is truncated and presumably not functional. The KF203 (↑lrp) mutant was constructed similarly using plasmid pKF203.
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Schwaiger et al. BMC Molecular Biology 2010 11:40 doi:10.1186/1471-2199-11-40