Additional file 4.

Premature truncated kunitz type SPI from B. flaviceps. Comparison of protein and nucleotide of truncated kunitz type SPI from B. flaviceps with EU246693 from Ophiophagus hannah. Exons are highlighted with different colors, Exon I is highlighted with red color, Exon II with Blue and Exon III is in grey color. 87 Nucleotides are deleted from the exon II of BF539 as shown with dashes in the figure. Comparison of the mRNA sequence of BF539 with BF294 reveals that a dinucleotide "GT" (underlined and highlighted in red letter) is present at the end of the exon II of BF539. The splicing error could be due to change in this base substitution. However the exon III is intact as stop codon and one of the amino acid residue is encoded by the exon III.

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Siang et al. BMC Molecular Biology 2010 11:24   doi:10.1186/1471-2199-11-24