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Open Access Research article

Transcriptomic analysis of the venom gland of the red-headed krait (Bungarus flaviceps) using expressed sequence tags

Ang Swee Siang1, Robin Doley12, Freek J Vonk3 and R Manjunatha Kini14*

Author Affiliations

1 Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Road, Singapore 117546, Singapore

2 Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur-784 028, Assam, India

3 Institute of Biology, Leiden University, Sylvius Laboratory, Sylviusweg 72, 2333 BE, Leiden, The Netherlands

4 Department of Biochemistry and Molecular Biology, Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia 23298-0614, USA

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BMC Molecular Biology 2010, 11:24  doi:10.1186/1471-2199-11-24

Published: 29 March 2010

Additional files

Additional file 1:

Transcripts submitted to database showing similarity to snake venom protein family. Numbers of transcripts, accession number, search programme and E values are shown in the table.

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Open Data

Additional file 2:

% of venom proteins families observed in venom gland transcriptome. Comparison of different toxin families observed in transcriptome of elapid and viperid venom gland.

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Additional file 3:

ASSET in 3FTxs of B. flaviceps. Alignment of 3FTx of B. flaviceps showing Accelerated Segment Switch in Exon to alter Targeting (ASSET). The segments which are similar are shown in same color where as segments which are dissimilar are shown in different color. The clone name and the number of clones are also shown in the figure.

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Additional file 4:

Premature truncated kunitz type SPI from B. flaviceps. Comparison of protein and nucleotide of truncated kunitz type SPI from B. flaviceps with EU246693 from Ophiophagus hannah. Exons are highlighted with different colors, Exon I is highlighted with red color, Exon II with Blue and Exon III is in grey color. 87 Nucleotides are deleted from the exon II of BF539 as shown with dashes in the figure. Comparison of the mRNA sequence of BF539 with BF294 reveals that a dinucleotide "GT" (underlined and highlighted in red letter) is present at the end of the exon II of BF539. The splicing error could be due to change in this base substitution. However the exon III is intact as stop codon and one of the amino acid residue is encoded by the exon III.

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Additional file 5:

Phylogenetic relationship of B chain and Kunitz SPI of different Bungarus species. Kunitz type SPI and B chain of β-bungarotoxin of Bungarus sp was obtained from the database and phylogenetic tree was constructed to understand the relationship between kunitz SPI and B chain of β-bungarotoxin.

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Additional file 6:

Comparison of BF95 from B. flaviceps with Laticauda semifasciata PLA2(AB062439). Exons are highlighted with different colors, Exon I is highlighted in green color; Exon II in magenta; Exon III in dark blue and Exon IV in grey. In BF95, part of the exon II (99 bp) is missing as shown in the figure with dashes.

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Additional file 7:

CRISPs and C-type lectins. A) Transcripts encoding CRISPs (BF53), found in this venom gland cDNA library. B) C-type lectins (BF53) found in this venom gland cDNA library. One of the C-type lectin found in this venom gland cDNA library was truncated in the 5' end (BF764). The amino acid residues of C-type lectin involved in binding to the Ca2+ are highlighted with green color and the cysteine residues are highlighted.

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