Table 2

Escherichia coli strains and plasmids used in this work

Strain or plasmid

Relevant characteristics

Reference/source

Strains

N3433

HfrHlacZ43 relA1 spoT1 thi-1

D. Apirion

N3431

N3433 rne3071(ts)

D. Apirion

IBPC633

N3433 rnc105

[34]

IBPC903

N3433 ΔpcnB(kan^R)

[58]

IBPC927

IBPC633 hfq1::Ω(kan^R, BclI)

this work

IBPC928

N3431 hfq1::Ω(kan^R, BclI)

this work

IBPC929

N3433 hfq1::Ω(kan^R, BclI)

[16]

IBPC941

N3433 hfqV43R cycA30::Tn10

[16]

IBPC953

N3433 hfqΔ22-294 cycA30::Tn10

[16]

IBPC981

IBPC903 hfqΔ22-294 cycA30::Tn10

this work

ENSO

HfrG6lacZΔ12

[56]

IBrpsO188::lacZ(*)

ENSO rpsO'-'lacZ

this work

LABrpsB208ΔGGGU(*)

ENSO rpsB'-'lacZ bearing deletion in

rpsB TIR from -72 to -69

[43]

Plasmids

pΔS15AUG

pCL1921 derivative expressing ΔrpsO

[38]

pACYC184

Tet^r, Cm^r

[59]

pS15

pACYC184 derivative expressing rpsO

this work

pGEM3

Amp^r

Promega corp.

pTX367 (**)

pGEM3 derivative expressing hfq

[5]

pTX381 (**)

pACYC184 derivative expressing hfq

[5]

(*) Tetracyclin-resistant derivatives of these strains, bearing cycA30::Tn10 and hfqΔ22-294 cycA30::Tn10, were constructed for using in this work. (**) These two plasmids contain the same insert. They were used without distinction assuming that the same amount of Hfq is overproduced due to autoregulation [16,17].

Le Derout et al. BMC Molecular Biology 2010 11:17   doi:10.1186/1471-2199-11-17