Hfq is not implicated in autogeneous repression of the rpsO mRNA by S15. (A) β-Galactosidase activities of the rpsO'-'lacZ chromosomal fusion in the hfq+ and hfqΔ derivatives of strain IBrpsO188::lacZ (Materials and methods) carrying plasmids pACYC184 (p) and its derivative pS15 expressing the rpsO gene. (B) Northern-blot analysis of the rpsO mRNA in strain IBrpsO188::lacZ transformed with pACYC184 (p) and its derivatives pS15 and pHfq (pTX381). Lower panel shows the amount of 5S RNA per lane. RNA was extracted from cells grown at 37°C to OD600≈0.4 in LB supplemented with chloramphenicol (34 μg/μl).
Le Derout et al. BMC Molecular Biology 2010 11:17 doi:10.1186/1471-2199-11-17