The relative expression level of EF, GAPDH and FEHII in chicory leaf and root. (a) EF normalised by ACT, (b) GAPDH normalised by ACT, (c) FEHII normalised by combined normalisation factor using ACT and EF and (d) FEHII normalised by GAPDH in young green leaf (L) and root tissues (R) of five wild type (Wt) chicory plants. Data are obtained from two independent RNA extractions for leaf tissue and two independent cDNA syntheses for R1 and R2 tissues. Expression profiles are calculated in qBase using selected reference genes and the gene specific amplification efficiency. Wt4-R1-1 as calibrator, ND: FEHII transcript was not detected in respective samples. Error bars represent standard error of the mean (white bars L samples, gray bars R1 samples and black R2 samples).
Maroufi et al. BMC Molecular Biology 2010 11:15 doi:10.1186/1471-2199-11-15