Table 5

Copies of Fluc normalized to the copies of the reference genes Rluc, B2M and ActB

Fluc/Rluc

Fluc/B2M

Fluc/ActB

Normalized copies

± SD

Normalized copies (× 10-3)

± SD

Normalized copies (× 10-3)

± SD


pMN85

Untreated

3.91

0.53

2.73

0.34

1.41

0.23

+ Cd

5.11

1.00

2.85

0.46

1.86

0.25

pMN86

Untreated

3.50

0.51

2.22

0.43

1.22

0.31

+ Cd

211.35

19.46

155.77

29.88

86.30

12.42


The number of copies of the genes (Fluc/Rluc/B2M/ActB) was calculated using standard curves generated as described in the Methods. The normalized copy numbers for Fluc/B2M and Fluc/ActB were multiplied by a factor of (1 × 103) to allow the comparison of the ratio of Fluc normalized to the chromosomal reference genes (B2M and ActB) to the ratio of Fluc normalized to the transiently co-transfected gene (Rluc). Abbreviations: SD- standard deviation

Jiwaji et al. BMC Molecular Biology 2010 11:103   doi:10.1186/1471-2199-11-103

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