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Open Access Highly Accessed Research article

Validation of reference genes for quantitative expression analysis by real-time RT-PCR in Saccharomyces cerevisiae

Marie-Ange Teste123, Manon Duquenne1234, Jean M François123 and Jean-Luc Parrou123*

Author Affiliations

1 CNRS, UMR5504, F-31400 Toulouse, France

2 INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France

3 Université de Toulouse; INSA, UPS, INP; LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France

4 Current address: Unité des Bactéries Lactiques et pathogènes Opportunistes, INRA - Centre de Recherche de Jouy-en-Josas, Domaine de Vilvert, 78352 Jouy-en Josas, France

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BMC Molecular Biology 2009, 10:99  doi:10.1186/1471-2199-10-99

Published: 30 October 2009

Additional files

Additional file 1:

Growth curve and glycogen content of WT strain on glucose and galactose. Growth (cells) and glycogen content during cultures of KT strain on glucose (set B from Figure 2) and galactose (set C from Figure 2). Cell samples (red dots) analyzed by real-time RT-PCR and sample numbering (S# followed by red numbers in the blue area). Cells (OD600), Glycogen (μg eq.glucose/OD unit).

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Additional file 2:

Growth curve and glycogen content of WT and tps1 strains. Growth (cells) and glycogen content during cultures of CEN.PK strains on galactose, WT (set D from Figure 2) and tps1 (set H from Figure 2). Legend as in Additional file 1.

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Additional file 3:

Ranking of reference genes according to their expression stability. Compiled data from all sample sets. For each set (A to M), genes are ranked from the least stable (left) to the most stable (right). The two most stable genes cannot be ranked in order. Gene expression stability value (Upper panel) as a function of gene name (lower panel).

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