Additional file 4.
RT-qPCR primer efficiency plots. Mean quantification cycle (Cq) values of each set of ten-fold serial dilution plotted against the logarithm of cDNA template concentration. The reaction efficiency (E) is given by [10(1/-S)-1] × 100%, where S represents the slope of the linear regression line.
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Hu et al. BMC Molecular Biology 2009 10:93 doi:10.1186/1471-2199-10-93