Table 4

Predicted protein sizes and antibody specificity

Variant

Size (aa)*

Predicted MWt (kDa)

Predicted expression (real-time PCR)

HASM

HBEC

BEAS2B

PBMC

PMN

THP1


Classical

283

31.5

++

++

+

+++

+++

+

Alternative Exon7

259

28.9

+++

+++

++

+++

-

++


Classical del3

235

26.2

-

-

-

++

++

-

Classical del5+6

189

21.2

-

-

-

++

++

-

Classical del6

234

26.1

++

++

+

+++

+++

+


Alternative del 4+5

160

17.8

+++

+++

++

++

-

+


D1 fragment

83/89

9.8

++

++

++

++

++

++

Classical D2/3

196

21.8

++

++

+

++

++

+

Alternative D2/3

172

19.2

++

++

++

++

-

++


* After removal of signal peptides

Predicted protein translations were performed for variants detected in real-time PCR assays (Figures 4 and 5) and predicted molecular weights (MWt, kDa) obtained. Note that these are potentially about 10 kDa smaller than might be detected at the protein level due to glycosylation. For each variant, relative expression based on real-time PCR results is summarised where +++: highest expression, ++: expression, + low expression and -: not detected. Other variants were detected in RACE experiments, which may result in expression of additional protein variants. Note that the D1 antibody would detect all variants except exon 3 deletions and D2/3 fragments, whilst the D2 antibody would not detect exon 4 deletions or the D1 fragment.

Stewart and Sayers BMC Molecular Biology 2009 10:75   doi:10.1186/1471-2199-10-75

Open Data