Table 3

Plasmids used in this study

Plasmid

Genotype/Description

Reference

pMalc2

ptac, Ap^R, ori ColE1, malE, lacZα, lacI^q, E. coli-Vektor for protein purification

NEB, Schwalbach

pMalc2amtR

pMalc2, p_tac-malE-amtR

This work

pMalc2amtR *Glu23Ala

pMalc2amtR, point mutation in amtR for AmtR*Glu23Ala variant

This work

pMalc2amtR* Arg52Ala

pMalc2amtR, point mutation in amtR for AmtR*Arg52Ala variant

This work

pMalc2amtR* Gly50Ala

pMalc2amtR, point mutation in amtR for AmtR*Gly50Ala variant

This work

pMalc2amtR* Ile51Ala

pMalc2amtR, point mutation in amtR for AmtR*Ile51Ala variant

This work

pMalc2amtR* Thr40Ala

pMalc2amtR, point mutation in amtR for AmtR*Thr40Ala variant

This work

pMalc2amtR* Thr42Ala

pMalc2amtR, point mutation in amtR for AmtR*Thr42Ala variant

This work

pMalc2amtR*Glu30Ala

pMalc2amtR, point mutation in amtR for AmtR*Glu30Ala variant

This work

pMalc2amtR*Gly36Ala

pMalc2amtR, point mutation in amtR for AmtR*Gly36Ala variant

This work

pMalc2amtR*Leu31Ala

pMalc2amtR, point mutation in amtR for AmtR*Leu31Ala variant

This work

pMalc2amtR*Phe32Ala

pMalc2amtR, point mutation in amtR for AmtR*Phe32Ala variant

This work

pMalc2amtR*Thr33Ala

pMalc2amtR, point mutation in amtR for AmtR*Thr33Ala variant

This work

pMalc2amtRAla54Gly

pMalc2amtR, point mutation in amtR for AmtR*Ala54Gly variant

This work

pMalc2amtRHis43Ala

pMalc2amtR, point mutation in amtR for AmtR*His43Ala variant

This work

pMalc2amtR*Gly36Ala

pMalc2amtR, point mutation in amtR for AmtR*Gly36Ala variant

This work

pMalc2amtR*Thr40Ala

pMalc2amtR, point mutation in amtR for AmtR*Thr40Ala variant

This work

pMalc2amtR*Thr42Ala

pMalc2amtR, point mutation in amtR for AmtR*Thr42Ala variant

This work

pMalc2amtR*Gly50Ala

pMalc2amtR, point mutation in amtR for AmtR*Gly50Ala variant

This work

pMalc2amtR*Ile51Ala

pMalc2amtR, point mutation in amtR for AmtR*Ile51Ala variant

This work

pMalc2amtR*Arg52Ala

pMalc2amtR, point mutation in amtR for AmtR*Arg52Ala variant

This work

pMalc2amtR*Gln53Ala

pMalc2amtR, point mutation in amtR for AmtR*Glu53Ala variant

This work

pMalc2amtR*Ser55Ala

pMalc2amtR, point mutation in amtR for AmtR*Ser55Ala variant

This work

pMalc2amtR*Leu56Ala

pMalc2amtR, point mutation in amtR for AmtR*Leu56Ala variant

This work

pMalc2amtR*Tyr57Ala

pMalc2amtR, point mutation in amtR for AmtR*Tyr57Ala variant

This work

pMalc2amtR*Tyr58Ala

pMalc2amtR, point mutation in amtR for AmtR*Tyr58Ala variant

This work

pMalc2amtR*His59Ala

pMalc2amtR, point mutation in amtR for AmtR*His59Ala variant

This work

pMalc2amtR*Leu70Ala

pMalc2amtR, point mutation in amtR for AmtR*Leu70Ala variant

This work

pMalc2amtR*Leu71Ala

pMalc2amtR, point mutation in amtR for AmtR*Leu71Ala variant

This work

Muhl et al. BMC Molecular Biology 2009 10:73   doi:10.1186/1471-2199-10-73