Table 1

Description of peach reference genes for RT-qPCR

Namea

Peach EST database accession number

Arabidopsis homolog locusb

Arabidopsis locus description

Function

Identities (%)


18S rRNA

TC1229

AT3G41768

18S ribosomal RNA

Cytosolic small ribosomal subunit, translation

97

ACT

TC1223

AT5G09810

Actin 2/7

Structural constituent of cytoskeleton

85

CYP2

TC1916

AT3G63400

Cyclophilin (CYP2)

Protein folding, RNA splicing

87

TEF2

TC3544

AT1G56070

Translation enlongation factor 2

Translation factor activity, nucleic acid binding

100

GAPDH

TC3113

AT1G13440

Glyceraldehyde-3-phosphate dehydrogenase

glycolysis

84

PLA2

DY636283

AT2G19690

Phospholipase A2 beta

Phospholipid metabolic process

71

RP II

TC1717

AT2G15430

RNA polymerase subunit

DNA-directed RNA polymerase activity, DNA binding

73

RPL13

TC5178

AT5G23900

60S ribosomal protein L13 (RPL13D)

Structural constituent of ribosome

100

TUA

TC2873

AT5G19780

Tublin alpha-5

Cytoskeleton structural protein

100

TUB

TC3624

AT1G75780

Tublin beta-1

Unidimensional cell growth, response to light stimulus

100

UBQ10

TC2782

AT4G05320

Ubiquitin 10

Protein modification process, protein binding

83


All peach ESTs were named based on similarity to Arabidopsis proteins determined via BLASTX. b Closest Arabidopsis homolog identified using TAIR BLAST [64]. AGI proteins database was queried with peach nucleotide sequences using BLASTX or in the case of 18S rRNA, Arabidopsis genome database with BLASTN.

Tong et al. BMC Molecular Biology 2009 10:71   doi:10.1186/1471-2199-10-71

Open Data