Additional file 1.
GATA-1 ChIP of the myb promoter. A) Location of ChIP primers in the myb promoter. B) Comparison of different derivatives of immobilized streptavidin: NeutrAvidin, streptavidin agarose, streptavidin mutein and M280 Dynabeads. Relative enrichment is calculated over non-transfected BirA control cells. C) The effects of preclearing chromatin and using 1% FGEL in blocking the beads. Enrichment was calculated relative to negative primer set. D) The effect of omitting SDS from the sonication buffer. Relative enrichment is calculated over non-transfected BirA control cells.
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Kolodziej et al. BMC Molecular Biology 2009 10:6 doi:10.1186/1471-2199-10-6