A) Location of the ChIP primers in the EKLF gene. "GATA" boxes indicate GATA-1 binding sites. B) Comparison of different derivatives of immobilized streptavidin: NeutrAvidin, streptavidin agarose, streptavidin mutein and M280 Dynabeads. Relative enrichment for EKLF sequences was calculated over negative control chromatin isolated from cells expressing BirA biotin ligase, but not tagged GATA-1.
Kolodziej et al. BMC Molecular Biology 2009 10:6 doi:10.1186/1471-2199-10-6